Popis: |
When glucose is added to the culture medium, some cells of the undifferentiated HT 29 line derived from a human colonic adenocarcinoma develop spherical structures which are bordered with microvilli and contain osmiophilic substance. These lumina, demonstrated as intracellular by the Ruthenium red method, in a preceeding paper, seem to originate from modifications of Golgi apparatuses such as dilatation and fusion of the stacks and genesis of microvilli which evaginate from cytoplasmic strands and present filamentous axes related with the network of intermediate filaments surrounding the lumina. |