Amino acid sequence of beta-galactosidase. VI. Limited tryptic digestion of the citraconylated protein and sequences of tryptic peptides

Autor:	A J Brake, Audree V. Fowler, Irving Zabin
Rok vydání:	1978
Předmět:	Chromatography biology Butanol Extraction (chemistry) Tryptic peptide Cell Biology Trypsin Biochemistry Hydrolysis chemistry.chemical_compound chemistry medicine biology.protein Beta-galactosidase Digestion Molecular Biology Peptide sequence medicine.drug
Zdroj:	Journal of Biological Chemistry. 253:5490-5498
ISSN:	0021-9258
DOI:	10.1016/s0021-9258(17)30400-3
Popis:	Hydrolysis with trypsin of citraconyl-carboxy-methyl-beta-galactosidase was carried out under limiting conditions. No Asp-Arg-X sequences were cleaved and many large peptides were produced. Butanol extraction from dilute acid proved very useful for separating the more hydrophobic fragments. Peptides were purified and sequenced. From this digest and two earlier preparations, all 80 theoretically possible tryptic fragments have been isolated and their structures determined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52f38ced3e4f25c1f5df07ce934a87f4 https://doi.org/10.1016/s0021-9258(17)30400-3 Zobrazit plný text záznamu