Competitive idiotype--anti-idiotype immunoassay for adenosine deaminase binding protein in urine

Autor:	M C Barrett, M J Nelles, Nina Tolkoff-Rubin, W. P. Hansen, C R Hewitt, R E Thompson, Robert H. Rubin, D J Piper
Rok vydání:	1985
Předmět:	Idiotype genetic structures biology medicine.diagnostic_test medicine.drug_class Chemistry Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Monoclonal antibody Molecular biology Adenosine deaminase Biochemistry Antigen Immunoassay Monoclonal biology.protein medicine Antibody
Zdroj:	Clinical Chemistry. 31:1833-1837
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/31.11.1833
Popis:	This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52f1071fa7e0bdb931a9b34b1bb06176 https://doi.org/10.1093/clinchem/31.11.1833 Zobrazit plný text záznamu