Raman Spectral Imaging Based Histopathology of Human Skin Tissue
| Autor: | Kaige Wang, Shuang Wang, Yaning Yin, Qingli He, Jie Qin, Xu Zhang, Jie Li, Jintao Bai, Yuze Gong |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
integumentary system Chemistry Confocal 010401 analytical chemistry Human skin Condensed Matter Physics 01 natural sciences 0104 chemical sciences Spectral imaging 010309 optics Melanin symbols.namesake medicine.anatomical_structure Dermis 0103 physical sciences symbols medicine Stratum corneum Biophysics Epidermis Raman spectroscopy Spectroscopy |
| Zdroj: | Journal of Applied Spectroscopy. 85:573-579 |
| ISSN: | 1573-8647 0021-9037 |
| Popis: | Confocal Raman microspectral imaging (CRMI) provides a versatile tool to extract biochemical information on histologically defined regions with improved spatial resolution and sensitivity. Here, we investigate spectral features and perform both uni- and multivariate analyses to obtain precise correlation between the biochemical profile and histological architecture of ex vivo human skin tissue. The skin dermis layer is visualized by integrated images of collagen peaks at 855, 940, and 1248 cm−1, while the stratum corneum layer is described by chemical images of ceramides. M elanin accumulation patterns in epidermis are obtained by integration of 1375 and 1580 cm−1 Raman peaks. In addition, distinct spectral features of the melanin precursor are revealed by certain Raman vibrations, which structurally characterize the molecular composition and organization of hair follicles. These results offer a unique insight into the skin histology, biochemistry, and biophysics, which provide the possibility of interpreting the underlying mechanisms of skin malignant processes. |
| Databáze: | OpenAIRE |
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