[31P]NMR measurements of hexokinase activity in intact red blood cells with 2-deoxyglucose as substrate

Autor:	J. Fonroget, Gérard Goethals, F. Halabi, J. P. Seguin
Rok vydání:	1988
Předmět:	chemistry.chemical_classification Hexokinase activity Hexokinase Chemistry Deoxyglucose General Engineering Substrate (chemistry) Nuclear magnetic resonance spectroscopy Red blood cell chemistry.chemical_compound medicine.anatomical_structure Enzyme Biochemistry Time course medicine
Zdroj:	Spectrochimica Acta Part A: Molecular Spectroscopy. 44:699-703
ISSN:	0584-8539
DOI:	10.1016/0584-8539(88)80130-2
Popis:	[ 31 P] NMR spectroscopy is demonstrated to be a suitable tool to follow the time course of 2-deoxyglucose-6-phosphate in intact human erythrocytes incubated with 2-deoxyglucose. It allowed to determine hexokinase V max and K m in near physiological conditions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52e6ffcab707ef1a08fa617ded21f39a https://doi.org/10.1016/0584-8539(88)80130-2 Zobrazit plný text záznamu