Growth Inhibitory Effects of Interferon-β but Not Interferon-α on Human Glioma Cells: Correlation of Receptor Binding, 2′,5′-O1igoadenylate Synthetase and Protein Kinase Activity
| Autor: | Ernest C. Borden, W. K. Alfred Yung, Peter J. Kelleher, Michael G. Rosenblum, Peter A. Steck, Frank J. Ruzicka |
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| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Cell growth Immunology Interferon beta-1a Alpha interferon Biology Molecular biology chemistry.chemical_compound Endocrinology chemistry Cell surface receptor Cell culture Virology Internal medicine medicine Growth inhibition Interferon alfa Interferon type I medicine.drug |
| Zdroj: | Journal of Interferon Research. 10:141-151 |
| ISSN: | 0197-8357 |
| DOI: | 10.1089/jir.1990.10.141 |
| Popis: | The antiproliferative effects of human recombinant interferon-α (r IFN-α A) and interferon-β (rIFN-βser) were assessed in vitro against seven human glioma cell lines. Further analysis of one of these lines (EFC-2) in response to rIFN-αA demonstrated a minimum growth inhibition by day 6 of treatment, whereas a 50% inhibition of cell growth was observed with a dose of 50 U/ml of IFN-βser. No significant growth inhibition was seen by rIFN-αA at doses up to 500 U/ml. Addition of rIFN-αA to rIFN-βser-treated EFC-2 cells neither suppressed nor augmented the antiproliferative response to IFN-βser. The binding of 125I-labeled rIFN-αA or 125I-labeled rIFN-βser to EFC-2 cells was inhibited competitively by increasing concentrations of either unlabeled rIFN-αA or rIFN-βser. This suggests that the cellular receptors for both rIFN-αA and rIFN-βser appear to be intact and appear to bind both agents equally. Furthermore, incubation of EFC-2 cells for 72 h with either rIFN-αA or rIFN-βser resulted in an increase... |
| Databáze: | OpenAIRE |
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