Quantitative Analysis of Human Hair for Selenium(IV), Selenium(VI) and Total Selenium by Hydride-Generation Atomic Absorption Spectrometry

Autor: Xiaonan Dong, Yuzuru Nakaguchi, Keizo Hiraki
Rok vydání: 1997
Předmět:
Zdroj: Analytical Sciences. 13:195-198
ISSN: 1348-2246
0910-6340
DOI: 10.2116/analsci.13.195
Popis: Optimized procedures were established for the determination of total Se, organic Se, Se(IV), and Se(VI) in human hair by means of hydride-generation atomic absorption spectrometry (HGAAS), respectively. Using this method, the total Se content in a hair sample was determined through pretreating the sample with nitric acid-perchioric acid, and then reducing Se(VI) to Se(IV) with 6.0mol dm-3 hydrochloric acid; however, for individual Se(IV), Se(VI) or soluble Se, after the hair sample was immersed into water and disrupted ultrasonically for 8h at room temperature, 2, 3-diaminonaphthalene (DAN) reagent was added to extract Se(IV). The sum of Se(IV) and Se(VI) was determined by extracting Se(IV) after reducing all of the Se(VI) into Se(IV) with the addition of potassium bromide. Se(VI) was obtained by subtracting Se(IV) from the sum of Se(IV) and Se(VI). The content of organic Se in the hair sample was calculated by subtracting Se(IV)+Se(VI) from the total Se. The detection limit of the method was 0.3ngSe cm-3, and the average precision (relative standard deviation) was less than 5% (n=6).
Databáze: OpenAIRE
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