| Autor: |
A. S. Krajewski, Ruth F. Jarrett, Jacqueline A. Lowrey, Sarah E. M. Howie, Alice Gallagher |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Etiology of Hodgkin’s Disease ISBN: 9781461380054 |
| DOI: |
10.1007/978-1-4613-0339-8_15 |
| Popis: |
Severe combined immunodeficient (SCID) mice were transplanted with biopsy material from 18 cases of Hodgkin’s disease (HD) and 23 biopsies from non-Hodgkin’s lymphoma. Five of the HD (3 lymphocyte predominant, 1 nodular sclerosing and 1 mixed cellularity) and 7 of the non-Hodgkin’s lymphomas (3 centroblastic, 1 immunoblastic, 1 follicular lymphoma and 2 biopsies from a case of large cell anaplastic lymphoma) produced tumours in mice. All the SCID HD-derived tumours had a B-cell phenotype and expressed the Epstein-Barr virus (EBV) gene products, EBER RNA, EBNA-2 and LMP-1. Morphologically these tumours resembled polymorphous immunoblastic lymphomas. The EBV-positive tumours all expressed CD23 and CD43. All HD-derived SCID tumours showed clonal immunoglobulin heavy chain rearrangements. One tumour derived from a follicular lymphoma showed similar morphology and phenotype and an identical pattern of EBV gene expression to the HD-derived tumours. Three centroblastic and one immunoblastic lymphoma derived tumours were EBV-negative. These showed a near identical B-cell phenotype to original biopsies and showed identical immunoglobulin gene rearrangements to the original tumours. Tumours derived from the large cell anaplastic lymphoma showed two types of lymphoproliferation in SCID mice; first immunoblastic tumours expressing EBER RNA, EBNA-2 and LMP-1, with immunoglobulin gene rearrangements differing from those of the original tumour; secondly large cell anaplastic tumours showing EBER RNA with little or no LMP-1 or EBNA-2 expression, and immunoglobulin gene rearrangements identical to those of the original tumour. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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