Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry

Autor:	Takehiko Yamamura, Hiroshi Kinoshita, Shie Yoshida, Minori Nishiguchi, Harumi Ouchi, Nobuyuki Adachi, Takako Minami, Kiyoshi Matsui, Hajime Nishio, Motonori Takahashi
Rok vydání:	2011
Předmět:	Detection limit Chromatography Calibration curve Methamidophos Biochemistry (medical) Extraction (chemistry) Analytical chemistry Toxicology Mass spectrometry Pathology and Forensic Medicine chemistry.chemical_compound chemistry Tissue extracts Gas chromatography–mass spectrometry Acephate
Zdroj:	Forensic Toxicology. 29:159-162
ISSN:	1860-8973 1860-8965
DOI:	10.1007/s11419-011-0110-9
Popis:	A simple and reliable method to determine acephate and methamidophos in mammalian tissues is presented. The method includes solid-phase extraction of tissue extracts with active carbon cartridges followed by gas chromatography–mass spectrometry analysis. During the study, a matrix effect was observed especially at low concentrations of acephate and methamidophos in serum and in brain. To minimize the effect, we prepared calibration curves with relatively short ranges. The validation data, such as the linearity of calibration curves, limits of detection, and coefficients of variation for recovery rates, were generally satisfactory. The present method is useful for determination of acephate and methamidophos in mammalian tissues because of its simplicity and speed, keeping in mind the presence of the matrix effect.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52ab93f69299768f4acbfa9bdbb3c028 https://doi.org/10.1007/s11419-011-0110-9 Zobrazit plný text záznamu Full text from SpringerLink