The value of GeneXpert MTB/RIF in bronchoalveolar lavage fluid in the diagnosis of smear-negative pulmonary tuberculosis
| Autor: | Lian-Peng Wu, Ning Pan, Yueying Zhou, Shi Jichan, Zhengxing Wu, Mei Yanhong, Cheng Aiqiong, Xian-gao Jiang |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty GeneXpert MTB/RIF medicine.diagnostic_test business.industry 030106 microbiology General Medicine respiratory system bacterial infections and mycoses Gastroenterology 03 medical and health sciences 0302 clinical medicine Bronchoalveolar lavage Pulmonary tuberculosis Internal medicine medicine Smear negative 030212 general & internal medicine business Value (mathematics) |
| Zdroj: | Investigación Clínica. 62:28-36 |
| ISSN: | 2477-9393 |
| DOI: | 10.22209/ic.v62n1a03 |
| Popis: | This study aims to evaluate the diagnostic value of Xpert MTB/ RIF assay in bronchoalveolar lavage fluid (BALF) in subjects with smear-negative pulmonary tuberculosis. From January 2019 to December 2019, 197 patients with suspected pulmonary tuberculosis were recruited, and bronchoalveolar lavage fluid was collected for acid-fast staining smear, liquid culture of Mycobacterium combined drug sensitivity and Xpert MTB/RIF detection. The sensitivity, specificity, positive predictive value and negative predictive value of Xpert MTB/RIF in bronchoalveolar lavage fluid (BALF) were calculated with smear-negative pulmonary tuberculosis as the reference standard. The consistency of xpert MTB/RIF in the diagnosis of rifampicin resistance was evaluated, with the results of Mycobacterium liquid culture drug sensitivity test and drug sensitivity test as the gold standards. The results showed that among 197 suspected tuberculosis patients, 55 patients were not diagnosed with tuberculosis and 142 patients were diagnosed with smear-negative pulmonary tuberculosis. One hundred and twenty three cases (86.62%) were positive for Xpert MTB/ RIF in bronchoalveolar lavage fluid, 15 cases (10.56%) were positive by acid-fast staining smear method, and 88 cases (61.97%) were positive by the liquid culture method. The positive rate of Xpert MTB / RIF was 93.18% (82 / 88), which was higher than that of 75.93% (41 / 54) of the negative BALF mycobacterium culture (χ 2 = 8.598, P |
| Databáze: | OpenAIRE |
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