| Autor: |
Rizzo, Ambra, Donzelli, Sara, Girgenti, Vita, Sacconi, Andrea, Vasco, Chiara, Salmaggi, Andrea, Blandino, Giovanni, Maschio, Marta, Ciusani, Emilio |
| Rok vydání: |
2017 |
| DOI: |
10.6084/m9.figshare.c.3796966_d7.v1 |
| Popis: |
Ectopic expression of miR-195-5p induces accumulation of cells in G0/G1. a-b) Proliferation assay (a) and viability assay (b) in U87MG cells transfected with miR-107 mimic or control. Cells were collected and counted at the indicated time points. c) Typical experiment. U87MG were transfected with control (left) or with mimic miR-195-5p mimic (right) and cultured for the following 48Â h. Cell were then harvested, fixed in 80% ethanol, stained with PI and analysed by flow cytometry for DNA content (see methods). d) U87MG cells morphology upon miR-195-5p exogenous expression. e) qRT-PCR of miR-195-5p in U87MG cells depleted for miR-195-5p (inh miR-195-5p) and treated with BRV or LCM (IC20). (PPTX 3351 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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