THE IMPORTANCE OF THE TRICARBOXYLIC ACID CYCLE IN THE RESPIRATION OF NEUROSPORA CRASSA

Autor: Donald Boulter, W. G. Rothery, A. W. Bown
Rok vydání: 1962
Předmět:
Zdroj: New Phytologist. 61:41-43
ISSN: 1469-8137
0028-646X
DOI: 10.1111/j.1469-8137.1962.tb06271.x
Popis: Boulter and Hurst (i960) have shown that particle preparations made from the mycelium of Neurospora crassa carry out the usual tricarboxylic-acid cycle of reactions. Those results, however, do not show to what extent, if any, these reactions operate in the metabolism of the intact organism. The object of this work is to shoM' the importance or otherwise of the cycle in tissue respiration. N. crassa 5256A (Emerson) was maintained on malt agar; spore inocula were grown in a minimal medium similar to that used by Beadle and Tatum (1941) except that o.i °,j sucrose was used as the carbon source. During the growth period the medium was vigorously agitated and this method of growth gave much lower rates of endogenous respiration than material grown with the more usual sucrose concentration of 2 %. When 0.02 M malate, succinate or citrate was added to mycelium which had been grown in the above manner for 24 hours the rate of respiration was increased by about 80%. Table i shows that o.oi M arsenite or 0.02s M fluoroacetate inhibited the respiration of the mycelium of  . crassa 40% and that o.oi M malonate inhibited the respiration 25 %.
Databáze: OpenAIRE
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