Comparative investigations of the γH2AX foci forming in human lung fibroblasts incubated in media containing tritium-labeled thymidine or amino acids

Autor:	O. A. Kochetkov, A. A. Tsishnatti, Yu. А. Fedotov, E. I. Yashkina, D. V. Gur’ev, Andreyan N. Osipov, S. M. Rodneva, A. A. Osipov A. A. Osipov, N. Yu. Vorob’eva, A. A. Maximov
Rok vydání:	2021
Předmět:	Alanine chemistry.chemical_classification Mechanical Engineering Energy Engineering and Power Technology MRC-5 Management Science and Operations Research Amino acid chemistry.chemical_compound Biochemistry chemistry Glycine Proline Thymidine Incubation DNA
Zdroj:	Cell Technologies in Biology and Medicine. :166-170
ISSN:	2413-4678 1814-3490
Popis:	We compared the formation of γH2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γH2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3H-thymidine was more than 2-fold higher than under the influence of 3H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3H-labeled amino acids showed that 3H-alanine produced more pronounced effect that 3H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::529710093b4da32851a03620271b5623 https://doi.org/10.47056/1814-3490-2021-3-166-170 Zobrazit plný text záznamu