Identification of the principal selenium compounds in selenium-enriched natural sample extracts by ion-pair liquid chromatography with inductively coupled plasma- and electrospray ionization-mass spectrometric detection

Autor:	Marc Birringer, Peter C. Uden, Julian F. Tyson, Mihály Kotrebai, Eric Block
Rok vydání:	1999
Předmět:	inorganic chemicals Electrospray Chromatography Chemistry Electrospray ionization food and beverages chemistry.chemical_element Mass spectrometry High-performance liquid chromatography Analytical Chemistry Yeast extract Sample preparation Inductively coupled plasma Selenium
Zdroj:	Analytical Communications. 36:249-252
ISSN:	1364-5536 1359-7337
DOI:	10.1039/a902770j
Popis:	Selenium-enriched garlic and yeast sample extracts and digests were analyzed using ion-pair high performance liquid chromatography (HPLC) with on-line inductively coupled plasma-mass spectrometric (ICP-MS) and electrospray ionization-mass spectrometric (ESI-MS) detection. The principal selenium compounds in these samples were identified as selenomethionine, and Se-adenosyl-selenohomocysteine in yeast, and γ-glutamyl-Se-methyl-selenocysteine and possibly γ-glutamyl-selenomethionine in garlic. The compounds identified account for 85 and 90% of the total selenium content of the yeast and the garlic samples, respectively. On-line HPLC-ESI-MS selected ion chromatograms (SIC) and mass spectra of selenium compounds extracted from selenium enriched samples are presented. Limits of quantification (LOQ, defined as S/N = 10) for HPLC-ICP-MS were in the range 10–50 ng mL–1 Se in the injected extracts. LOQ values for HPLC-ESI-MS were ca. 100 times higher than those of HPLC-ICP-MS.
Databáze:	OpenAIRE
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