Molecular interaction during pregnancy: Biochemical and topological analysis of adult T-cell leukaemia-derived factor, homologous to thioredoxin, in the pregnant human uterus
| Autor: | Hiroshi Masutani, Takahide Mori, Junji Yodoi, Yuuki Kitaoka, Shingo Fujii, Yoshihiko Nanbu, Hajime Nakamura, Norimasa Sagawa, Fuminori Kobayashi |
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| Rok vydání: | 1995 |
| Předmět: |
Gel electrophoresis
medicine.medical_specialty Rehabilitation Decidua Obstetrics and Gynecology Trophoblast Biological activity macromolecular substances Biology Molecular biology medicine.anatomical_structure Endocrinology Reproductive Medicine Internal medicine Placenta embryonic structures medicine biology.protein Cytotoxic T cell Thioredoxin Antibody reproductive and urinary physiology |
| Zdroj: | Human Reproduction. 10:1603-1608 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/10.6.1603 |
| Popis: | Adult T-cell leukaemia-derived factor (ADF), homologous to thioredoxin, displays various biological activities, such as radical scavenging action and the reduction of protein disulphide bonds. We examined the biochemical and immunohistochemical localization of ADF in the pregnant human uterus, using two heteroantibodies to ADF, antibody C and W. Immunohistochemically, decidua and trophoblast cells were intensely stained by antibody C. The concentration of ADF-like substance in the decidua was 95.9 ng/mg protein, determined by enzyme-linked immunosorbent assay. The molecular weight of ADF-like substance in these tissues was determined by gel electrophoresis to be 13 kDa, the same as that of recombinant ADF. These findings indicate that abundant ADF is present in decidua and trophoblast cells ; the localization of such a potent dithiol reducing substance may be beneficial in protecting the fertilized egg and placental trophoblasts from the cytotoxic effects of oxygen radicals. |
| Databáze: | OpenAIRE |
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