Isolation and Identification of Pathogenic Acanthamoeba Species from Air Conditioning Systems, Egypt
| Autor: | Mohamed Mahmoud Abdel-Fatah, Rasha F. Ahmed, Ekhlas H. Abdel-Hafeez, Nisreen Abdel Tawab Abdel Gaber, Rabie M. Mohamed, Nabil Shokrany Gabr, Usama S. Belal |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Inoculation 030106 microbiology Acanthamoeba species General Medicine Biology medicine.disease Isolation (microbiology) biology.organism_classification Keratitis Acanthamoeba law.invention Microbiology 03 medical and health sciences 0302 clinical medicine Infectious Diseases law parasitic diseases medicine 030212 general & internal medicine Granulomatous amoebic encephalitis In vitro cell culture Polymerase chain reaction |
| Zdroj: | Japanese Journal of Infectious Diseases. 74:180-186 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2020.049 |
| Popis: | Acanthamoeba are free-living amoebae that cause granulomatous amoebic encephalitis and keratitis. In this study, we aimed to isolate and identify Acanthamoeba from air conditioning systems using in vitro cell culture and polymerase chain reaction assays. We also estimated the pathogenicity of the isolates by measuring their thermotolerance and studying mice models inoculated with these isolates. Of the 80 dust samples acquired, 41 (51.25%) were found to be positive for Acanthamoeba spp. using in vitro cell culture and the results were validated using PCR. Out of these 41 samples, 27 (65.9%) were thermotolerant and 16 (39%) samples could infect mice and cause histopathological effects. Highly pathogenic Acanthamoeba isolates were characterized by their thermotolerance and the ability to disseminate in all organs after infection, causing early death of infected animals. Our study thus validated the presence of pathogenic isolates of Acanthamoeba in air conditioners that may be potentially infectious to humans. |
| Databáze: | OpenAIRE |
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