Identification of guanosine 3′:5′-monophosphate in the fruit of Zizyphus jujuba
| Autor: | Jyong-Chyul Cyong, Makoto Takahashi |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Chromatography
biology Zizyphus jujuba food and beverages Guanosine Phosphodiesterase Ripening Plant Science General Medicine Horticulture biology.organism_classification Biochemistry High-performance liquid chromatography C++ AMP chemistry.chemical_compound Ultraviolet visible spectroscopy chemistry Rhamnaceae Molecular Biology |
| Zdroj: | Phytochemistry. 21:1871-1874 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/0031-9422(82)83005-7 |
| Popis: | Evidence is presented here confirming the identification of guanosine 3′: 5′-monophosphate (c GMP) in the tissue of higher plants. The c GMP activity detected in fruits of Zizyphus jujuba was separated from the c AMP activity also present. The separated sample was extensively purified by Bio-Rad AG 1 × 4 and aluminium oxide CC, and by TLC. The purified sample showed the same physicochemical properties as authentic c GMP by TLC using different solvents and by UV spectroscopy, and was decomposable by cyclic nucleotide-specific phosphodiesterase. The identification was further supported by HPLC. The amount of c GMP present increases 90-fold during fruit ripening. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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