Gemini-Type Tetraphenylethylene Amphiphiles Containing [12]aneN3 and Long Hydrocarbon Chains as Nonviral Gene Vectors and Gene Delivery Monitors
| Autor: | You-Di Shi, Zhong-Lin Lu, Bing Gong, Lin Song, Ai-Xiang Ding, Zheng-Li Tan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Liposome
Chemistry 02 engineering and technology Transfection Tetraphenylethylene Gene delivery 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Micelle 0104 chemical sciences chemistry.chemical_compound Amphiphile Biophysics Organic chemistry Moiety General Materials Science 0210 nano-technology DNA |
| Zdroj: | ACS Applied Materials & Interfaces. 9:11546-11556 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.7b01850 |
| Popis: | Four gemini amphiphiles decorated with triazole-[12]aneN3 as the hydrophilic moiety and various long hydrocarbons as hydrophobic moieties, 1-4, were designed to form micelles possessing the aggregation-induced emission (AIE) property for gene delivery and tracing. All four amphiphiles give ultralow critical micelle concentrations, are pH-/photostable and biocompatible, and completely retard the migration of plasmid DNAs at low concentrations. The DNA-binding abilities of the micelles were fully assessed. The coaggregated nanoparticles of 1-4 with DNAs could convert back into AIE micelles. In vitro transfections indicated that lipids 1 and 2 and their originated liposomes bearing decent delivering abilities have great potentials as nonviral vectors. Finally, on the basis of the transfection and the transitions between condensates and micelles, lipid 2 was singled out as the first example for real-time tracing of the intracellular deliveries of nonlabeled DNA, which provides spatiotemporal messages about the processes of condensate uptake and DNA release. |
| Databáze: | OpenAIRE |
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