Determination of metolachlor in water and soil by a rapid magnetic particle-based ELISA
| Autor: | James R. Fleeker, Charles E. Lachman, Fernando M. Rubio, Scott W. Jourdan, Timothy S. Lawruk, David P. Herzog |
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| Rok vydání: | 1993 |
| Předmět: |
Detection limit
Immunogen Chromatography medicine.diagnostic_test biology Chemistry Parts-per notation General Chemistry chemistry.chemical_compound Polyclonal antibodies Immunoassay medicine biology.protein Chloroacetamide Bovine serum albumin General Agricultural and Biological Sciences Metolachlor |
| Zdroj: | Journal of Agricultural and Food Chemistry. 41:1426-1431 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf00033a014 |
| Popis: | A competitive enzyme-linked immunosorbent assay (ELISA) for the quantitation of metolachlor in water and soil was developed using a magnetic particle-based solid phase. Metolachlor was covalently attached to bovine serum albumin carrier through the chloroacetamide moiety and the resulting herbicide-protein immunogen used in rabbits to produce polyclonal antibodies specific for metolachlor. The polyclonal antibodies are covalently attached to amine-terminated superparamagnetic particles and used as the solid phase in a rapid and sensitive immunoassay. This ELISA has a limit of detection of 0.05 ppb (parts per billion, nanograms per milliliter) in water and 3.0 ppb in soil. The ELISA compares favorably with GC measurements when water samples are analyzed (r=0.976) |
| Databáze: | OpenAIRE |
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