| Popis: |
A thorough understanding of how biomolecular interaction curves should look like is essential when evaluating surface plasmon resonance experimental data. This chapter discusses the shape of the biomolecular interaction curve, mentions crucial properties of the interaction curve and shows deviations from the ideal situation. In addition, the experimental conditions are discussed to avoid sub-optimal interaction curves and tests are presented to detect sub-optimal situations. Because the interaction kinetics can vary significantly between different interactants, experimental strategies that can be used are outlined for different situations. A small section is dedicated to the fitting of the curves and the validation of the results. Special attention is given to publishing sensorgrams. |
