Molecular detection ofSalmonellaserovars Enteritidis, Heidelberg and Typhimurium directly from pre-enriched poultry samples
| Autor: | Fernanda Kieling Moreira Lehmann, Margarida Neves Souza, S. De Carli, Vagner Ricardo Lunge, André Salvador Kazantzi Fonseca, Nilo Ikuta, Diéssy Kipper |
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| Rok vydání: | 2019 |
| Předmět: |
Serotype
Salmonella Veterinary medicine 040301 veterinary sciences 0402 animal and dairy science Outbreak 04 agricultural and veterinary sciences General Medicine Biology medicine.disease_cause 040201 dairy & animal science 0403 veterinary science medicine Animal Science and Zoology Diagnostic laboratory Flock Food Science |
| Zdroj: | British Poultry Science. 60:388-394 |
| ISSN: | 1466-1799 0007-1668 |
| DOI: | 10.1080/00071668.2019.1614525 |
| Popis: | 1. Salmonella is one of the most important pathogens in public health and it is usually associated with food-borne diseases. Salmonella serovars Enteritidis and Typhimurium are widespread in the world with outbreaks frequently associated with consumption of poultry products; furthermore, there is an increasing public health concern with the wide dissemination of the serovar Heidelberg in poultry flocks. 2. The aim of the experiment was to develop and to validate rapid methods to detect Salmonella serovars Enteritidis, Typhimurium, and Heidelberg by real-time PCRs and test isolates from pre-enriched poultry samples. 3. Three real-time PCRs were developed and used in combination to detect the serovars Enteritidis, Typhimurium and Heidelberg. These assays were validated by the analysis of 126 Salmonella isolates, eight other enteric bacterial species and 34 naturally contaminated poultry samples after pre-enrichment with buffered peptone water (BPW). 4. Real-time PCRs detected the isolates of the most important poultry serovars (Enteritidis, Typhimurium and Heidelberg) with 100% inclusivity and exclusivity in each assay. The PCR identified monophasic variants of the serovars Typhimurium and Heidelberg. All PCRs were validated in detecting these specific serovars directly from pre-enriched poultry samples. The whole analytical procedure was performed in less than 24 h in a veterinary diagnostic laboratory. |
| Databáze: | OpenAIRE |
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