Isolation and partial characterization of biologically active polysaccharides fromPlantago major L

Autor: Terje Espevik, Berit Smestad Paulsen, Jens K. Wold, Haruki Yamada, Hanako Otsuka, Anne Berit Samuelsen
Rok vydání: 1995
Předmět:
Zdroj: Phytotherapy Research. 9:211-218
ISSN: 1099-1573
0951-418X
DOI: 10.1002/ptr.2650090312
Popis: Polysaccharide fractions were isolated from Plantago Major L. leaves by extraction with cold water, hot water and with dimethylsulphoxide (DMSO). Each of the three crude extracts obtained was further separated by ion-exchange chromatography. Only the two acidic fractions from the 50°C crude extract were biologically active, demonstrated by in vitro testing of activation of the complement system and induction of tumour necrosis factor alpha (TNF-α) production from human monocytes. One of the fractions, PM I 50 , contains polysaccharide material comprising arabinose, galactose and xylose with smaller proportions of galacturonic acid, glucose, mannose and rhamnose. This fraction was further fractionated by size exclusion chromatography to give three fractions PM I a , PM I b and PM I c .PM I a is probably an arabinogalactan and PM I b is mainly a 1,4-linked xylan. The other biologically active fraction, PM II 50 is composed of 75% galacturonic acid, 3% rhamnose, 6% arabinose and 8% galactose. Structure analysis indicates that PM II 50 is a pectin type polysaccharide
Databáze: OpenAIRE
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