Development of a Rapid, Quantitative Method for LDL Subfractionation with Use of the Quantimetrix Lipoprint LDL System
| Autor: | John F. O'Brien, Irene Meissner, Daniel M. Hoefner, Shannon D. Hodel, Joseph P. McConnell, Earl L. Branum, Deborah Sun |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Gel electrophoresis
Chromatography Apolipoprotein B biology Triglyceride Cholesterol Biochemistry (medical) Clinical Biochemistry Nuclear magnetic resonance spectroscopy chemistry.chemical_compound chemistry biology.protein lipids (amino acids peptides and proteins) Densitometry Polyacrylamide gel electrophoresis Quantitative analysis (chemistry) |
| Zdroj: | Clinical Chemistry. 47:266-274 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/47.2.266 |
| Popis: | Background: Recent evidence suggests that the presence of small, dense LDL is independently associated with increased risk of developing coronary artery disease. Current methods to subfractionate LDL are time-consuming and/or technically demanding. Therefore, we have sought the development of a less complex LDL subfractionation procedure.Methods: LDL subfractions were separated using the Quantimetrix LipoprintTM LDL System. High-resolution 3% polyacrylamide gel tubes were scanned densitometrically (610 nm) with a Helena EDC system. A computerized method to identify and quantitatively score the resolved LDL subfractions was developed. Results from the Quantimetrix method were compared using 51 plasma samples with values obtained by nondenaturing gradient gel electrophoresis (NDGGE) and nuclear magnetic resonance (NMR) spectroscopy.Results: LDL subfractionation scores correlated significantly (P Conclusions: A quantitative method for the assessment of LDL particle size phenotype was developed using the Quantimetrix Lipoprint LDL System. The method can be performed in less than 3 h in batch mode and is suitable for routine use in clinical laboratories. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|