Removal of an endogenous antigen from an antibody to increase its effective affinity constant, as illustrated by triiodothyronine assay

Autor:	C Cano, L K Oliver
Rok vydání:	1977
Předmět:	Antiserum Ethanol Triiodothyronine biology Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Endogeny Molecular biology chemistry.chemical_compound Antigen chemistry biology.protein Antibody Hormone
Zdroj:	Clinical Chemistry. 23:2039-2042
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/23.11.2039
Popis:	Antisera to triiodothyronine were shown to contain large quantities of the hormone, well in excess of normal circulating concentrations. Extracting the triiodothyronine from the antiserum with alkaline ethanol yielded an antibody of increased affinity. Use of this antibody in a radioimmunoassay resulted in a fourfold increase in sensitivity as compared with the unextracted material.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::522c647ef1f28f6ccd4b4d92110009b4 https://doi.org/10.1093/clinchem/23.11.2039 Zobrazit plný text záznamu