CircMACF1 Attenuates Acute Myocardial Infarction Through miR-500b-5p-EMP1 Axis
Autor: | Zuoyan Wang, Jianjun Peng, Sheng Zhao, Bo Zhao, Lihui Ren, Huiming Ye, Guangping Li, Licheng Lei |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
TUNEL assay business.industry Pharmaceutical Science 030204 cardiovascular system & hematology medicine.disease Epithelial membrane protein-1 Pathogenesis 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Downregulation and upregulation chemistry Apoptosis Annexin Lactate dehydrogenase Genetics Cancer research Molecular Medicine Medicine Myocardial infarction Cardiology and Cardiovascular Medicine business Genetics (clinical) |
Zdroj: | Journal of Cardiovascular Translational Research. 14:161-172 |
ISSN: | 1937-5395 1937-5387 |
Popis: | It is widely accepted that circular RNA (circRNA) plays an important role in cardiovascular diseases. Therefore, this experiment aimed to investigate the pathogenesis of circMACF1 in acute myocardial infarction (AMI). qRT-PCR and immunoblotting were used to detect the expression levels of circMACF1, miR-500b-5p, and epithelial membrane protein 1 (EMP1). The role of circMACF1, miR-500b-5p, and EMP1 in cardiomyocyte apoptosis was assessed using annexin V-FITC/PI. Echocardiographic assessment, serum creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH), myocardial infarct size, and TUNEL staining were applied in our research. In the MI group, the expression levels of circMACF1 and EMP1 were decreased with the increasing expression level of miR-500b-5p. CircMACF1 upregulated the expression of EMP1 as a sponge of miR-500b-5p, and circMACF1 was a direct target of miR-500b-5p. CircMACF1 impaired the progression of AMI by modulating the miR-500b-5p/EMP1 axis. CircMACF1 may be a potential therapeutic target for treating AMI. Graphical Abstract CircMACF1 upregulated EMP1 expression by sponge miR-500b-5p. |
Databáze: | OpenAIRE |
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