The Long Noncoding RNA OIP5-AS1 Is Involved in the Regulation of Cell Proliferation
| Autor: | Toshiyuki Tsunoda, Yuuga Sawata, Senji Shirasawa, Madoka Naemura, Nagisa Arikawa, Masahide Kuroki, Yojiro Kotake |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Anticancer Research. 38 |
| ISSN: | 1791-7530 0250-7005 |
| Popis: | Background/aim OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation. Materials and methods HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis. Results OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten rat sarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G2/M phase cell-cycle arrest. Conclusion These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G2/M phase progression. |
| Databáze: | OpenAIRE |
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