ROS mediated pro-apoptotic effects of i Tinospora cordifolia i on breast cancer cells
| Autor: | Jamal Akhtar Ansari, Nishat Fatima, Mohammad Kaleem Ahmad, Mohammad Waseem, Abdul Rahman Khan, Abbas Ali Mahdi, Homa Jilani Khan, Vikas Kumar Srivastava, Ravi Thakur, Namrata Rastogi, Durga Prasad Mishra |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Tinospora cordifolia General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer Medicine skin and connective tissue diseases chemistry.chemical_classification Reactive oxygen species General Immunology and Microbiology biology business.industry Cell growth biology.organism_classification medicine.disease 030104 developmental biology chemistry Colony formation Apoptosis Cancer research Breast cancer cells business Quercetin 030217 neurology & neurosurgery |
| Zdroj: | Frontiers in Bioscience. 9:89-100 |
| ISSN: | 1945-0508 1945-0494 |
| DOI: | 10.2741/e788 |
| Popis: | The inevitable development of chemoresistance and unmanageable side effects are the major therapeutic challenges in management of breast cancer imposing an urgent need for identification of novel therapeutic agents. In the present investigation, we report anti-proliferative activity of chloroform fraction of Tinospora cordifolia (TcCF), an Ayurvedic medicinal plant, on breast cancer cells. We found that TcCF inhibited growth of breast cancer cells, MDA-MB-231 and MCF-7. More interestingly, we observed TcCF treatment increased intra-cellular ROS levels, altered expression of pro and anti-apoptotic genes, decreased colony formation ability and induced apoptosis in breast cancer cells. We also found that inhibition of ROS abrogated TcCF induced apoptosis in breast cancer cells, emphasizing the role ROS in TcCF induced breast cancer cell death. Furthermore, we identified the presence of pharmacologically active compounds like rutin and quercetin which account for the anti-cancer property of TcCF against breast cancer cells. These data show TcCF is a promising anti-cancer agent against breast cancer cells. |
| Databáze: | OpenAIRE |
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