A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma
Autor: | Rong Deng, Weiyi Chen, Hua-Zhong Yu, Xiaochun Li, Hou Pengfei, Jiqiang Guo |
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Rok vydání: | 2021 |
Předmět: |
Detection limit
Scanner Calibration curve Chromogenic Computer science 010401 analytical chemistry 02 engineering and technology Standard solution 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Blood plasma Multiplex 0210 nano-technology Colorimetry Biomedical engineering |
Zdroj: | Analytical and Bioanalytical Chemistry. 413:4625-4634 |
ISSN: | 1618-2650 1618-2642 |
Popis: | Herein we report a quantitative, multiplex assay for disease markers in plasma based on an integrated setup of a portable scanner and a disposable paper-based analytical device (PAD). The quantitative analysis relies on the digital colorimetric reading of the three-layer PAD with 30 assay sites for performing respective chromogenic reactions for plasma uric acid, glucose, and triglyceride, which are considered as important risk factors for cardiovascular diseases. A portable scanner with WiFi transmission capability was used to produce high-quality color images of the PADs and wirelessly transfer them to a smartphone or other mobile devices for data processing. The concentrations of biomarkers in both standard solutions and plasma samples can be directly obtained using a custom-designed smartphone app that is also capable of constructing calibration curves. The detection limits of uric acid, glucose, and triglyceride were determined to be 0.50 mg/dL, 0.84 mmol/L, and 14 mg/dL, respectively, which are below the normal limits and adequate for clinical validation. Owing to the distinct advantages—simple, portable, and cost-effective—this mobile assay protocol can be used for point-of-care (POC) settings or resource-limited situations, and potentially for the diagnosis and prevention of infectious diseases. |
Databáze: | OpenAIRE |
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