High Concentration of Iopromide Induces Apoptosis and Autophagy in Human Embryonic Kidney Cells via Activating a ROS-dependent Cellular Stress Pathway

Autor:	Yih-Dih Cheng, Fuu Jen Tsai, Yu-Jen Chiu, Shih-Chang Tsai, Yuh-Feng Tsai, Jai Sing Yang
Rok vydání:	2021
Předmět:	Pharmacology Cancer Research Programmed cell death Cell growth Chemistry HEK 293 cells Autophagy medicine.disease General Biochemistry Genetics and Molecular Biology Cell biology Apoptosis medicine Viability assay Protein kinase B Cell damage
Zdroj:	In Vivo. 35:3221-3232
ISSN:	1791-7549 0258-851X
Popis:	BACKGROUND/AIM The use of iodinated contrast media may impair renal function. However, no report has addressed the nephrotoxicity of high doses of iodinated contrast media in normal kidney cells and its associated molecular mechanisms. MATERIALS AND METHODS Cell proliferation was assessed using the MTT assay. Cell death was evaluated through examining the morphological changes and TUNEL assay. Autophagy was detected through acridine orange staining and lysotracker staining. Reactive oxygen species production and AKT kinase activity were examined. RESULTS Iopromide induced cell death and triggered apoptosis and autophagy in HEK 293 cells. Cell viability was significantly restored in the presence of a pan-caspase inhibitor or a ROS scavenger, N-acetyl-L-cysteine. AKT kinase activity was found to be reduced in iopromide-treated HEK 293 cells. CONCLUSION High concentrations of iopromide induce cell damage, apoptosis, and autophagy through down-regulating AKT and ROS-activated cellular stress pathways in HEK 293 cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::52190b31575e0d52eb241fceb711394a https://doi.org/10.21873/invivo.12617 Zobrazit plný text záznamu