Effects of Exogenous p53 Transduction in Thyroid Tumor Cells with Different p53 Status1
| Autor: | Simona Nanni, Fabiola Moretti, Michela Narducci, Ada Sacchi, Alfredo Pontecorvi, Antonella Farsetti, S Giuliacci, Marco Crescenzi |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Cell Thyroid Estrogen receptor Cell cycle Biology medicine.disease Biochemistry Thyroid carcinoma Transduction (genetics) Endocrinology medicine.anatomical_structure Cell culture Internal medicine medicine Cancer research Thyroid cancer |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 85:302-308 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.85.1.6295 |
| Popis: | Recovery of p53 function in undifferentiated thyroid carcinoma cells carrying an altered p53 gene is able to modify cell tumorigenic properties. It is not known whether such an effect may also be achieved in thyroid cancer cells expressing wild-type p53, as in the majority of differentiated thyroid carcinomas. Effects of p53 transduction in a thyroid carcinoma cell line (FRO) exhibiting a wild-type endogenous p53 gene, in comparison to a cell line (WRO) exhibiting mutant p53, were investigated by using an inducible chimeric construct containing human p53 complementary DNA fused to the ligand binding domain of the estrogen receptor (p53ER). FRO cells were unaffected by exogenous p53 expression in terms of both proliferation and viability. On the contrary, p53 reexpression in WRO cells containing hemizygous mutated p53 allele caused a strong growth inhibition due to cell accumulation in the G1 phase of the cell cycle. In addition, exogenous p53 did not influence FRO cell behavior in response to TSH treatmen... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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