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Backround: Dirofilaria species are important zoonotic filarioid nematodes transmitted by mosquitoes causing a heartworm disease in canines worldwide. Map of the distribution pattern of dirofilariois in Nepalese context is lacking. Methodology: A study was done to access the occurrence of dirofilarioids among stray dogs population in Siddharthanagar, sub-metropolitan city of Lumbini, Nepal. A total of 150 blood specimens were examined using direct microscopy, buffy coat centrifugation and modified Knott's method. Haematobiochemical parameters including packed cell volume (PCV), alanine aminotransferase (SGPT), aspartate aminotransferase (SGOT), alkaline phosphate (ALP), creatinine, blood urea nitrogen (BUN), urea and total protein were evaluated. Furhter, the clinical performance, comprising the body temperature and respiratory rate, were also assessed. Results: The dirofilariod is recognized as Dirofilaria immitis . Overall prevalence of dirofilariosis was reported to be 19.33% (29/150). Meanwhile, microfilariae were noticed to be 16.0, 16.0 and 19.33 % using the direct blood smear microscopy, buffy coat and modified Knott technique, respectively. The infection rate was significantly ( P |
