Specific primers for the detection of vip3A insecticidal gene within aBacillus thuringiensiscollection

Autor:	W.C. Rice
Rok vydání:	1999
Předmět:	Genetics Sequence analysis fungi Biology Amplicon biology.organism_classification Applied Microbiology and Biotechnology law.invention Restriction enzyme law Complementary DNA Bacillus thuringiensis Primer (molecular biology) Gene Polymerase chain reaction
Zdroj:	Letters in Applied Microbiology. 28:378-382
ISSN:	1472-765X 0266-8254
DOI:	10.1046/j.1365-2672.1999.00536.x
Popis:	A PCR-based method was developed for the rapid detection of vip3A gene encoding a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Specific primer combinations (three primers for the normal strand and two primers for the complementary strand) were capable of generating diagnostic fragments that successfully predicted the presence of the gene encoding the Vip3A insecticidal toxin in various B. thuringiensis strains. Specificity of amplicons generated by primer pairs was confirmed by restriction endonuclease digestion and DNA sequence analysis. The evaluation of B. thuringiensis strains for biological activity against insect pests of rice is thus aided by the grouping of strains based on their potential insecticidal spectrum.
Databáze:	OpenAIRE
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