| Autor: |
Anupriya Aggarwal, Alberto Stella, Anouschka Akerman, Gregory Walker, Vanessa Milogiannakis, Samantha McAllery, Camille Esneau, Mariana Ruiz Silva, Yonghui Lu, Charles Forster, Sebastiaan van Hal, Vennila Mathivanan, Christina Fitcher, Alexandra Carey Hoppe, C. Mee Ling Munier, David Darley, Gail Matthews, Damien Starck, Roselle Robosa, Nathan Bartlett, William Rawlinson, Anthony Kelleher, Stuart Turville |
| Rok vydání: |
2022 |
| Popis: |
From late 2020 the world observed the rapid emergence of many distinct SARS-CoV-2 variants. At the same time, pandemic responses coalesced into significant global vaccine roll-out that have now significantly lowered Covid-19 hospital and mortality rates in the developed world. Over this period, we developed a rapid platform (R-20) for viral isolation and characterisation using primary remnant diagnostic swabs. This combined with quarantine testing and genomics surveillance, enabled the rapid isolation and characterisation of all major SARS-CoV-2 variants (all variants of concern and 6 variants of interest) globally with a 4-month period. This platform facilitated viral variant isolation and enabled rapid resolution of variant phenotype by allowing determining end point viral titers from primary nasopharyngeal swabs and through ranking of evasion of neutralising antibodies. In late 2021, when the Delta variant was dominating, Omicron rapidly emerged. Using this platform, we isolated and tested the first cases of this variant within Australia. In this setting we observed Omicron to diverge from other variants at two levels: Firstly, it ranks at the mots evasive to neutralisation antibodies compared to all VOCs and major VUIs. Secondly, it no longer engages TMPRSS2 during the late stages of fusion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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