Induction of ectodermal cells from vegetal-endodermal blastomeres of amphioxus (Branchiostoma belcheri tsingtaunese) embryos by the calcium ionophore A23187

Autor:	Zhang Hong-wei, Zhang Shi-cui, Mao Bing-yu, Zheng Jia-sheng
Rok vydání:	1999
Předmět:	Genetics animal structures Epidermis (botany) chemistry.chemical_element Embryo Blastomere Biology Calcium Oceanography Cell morphology Cell biology Gastrulation medicine.anatomical_structure chemistry embryonic structures medicine Endodermis Endoderm Water Science and Technology
Zdroj:	Chinese Journal of Oceanology and Limnology. 17:97-104
ISSN:	1993-5005 0254-4059
DOI:	10.1007/bf02842706
Popis:	Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively tested effects of A23187, the calcium ionophore, on the development of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal blastomeres committed to endoderm were treated with A23187 prior to gastrulation, they were transformed into ectodermal cells as evidenced by the cell morphology and function characteristic of epidermis. However, the developmental fate of the same blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged. Thus, vegetal-endodermal cells in amphioxus embryos are not irreversibly determined before the gastrula stage, and artificial increase in intracelluar Ca2+ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::51e52f3910543ec1be2a6f4e5d419b2f https://doi.org/10.1007/bf02842706 Zobrazit plný text záznamu Full text from SpringerLink