Differential axial contrast of optical sections: laser microtomography and quantitative 3D reconstruction

Autor:	M. A. Pogorelova, A. G. Pogorelov, V. A. Golichenkov
Rok vydání:	2014
Předmět:	Microscope Materials science business.industry 3D reconstruction Image processing Laser Atomic and Molecular Physics and Optics Displacement (vector) Electronic Optical and Magnetic Materials law.invention Optical axis Superposition principle Optics law Photonics business
Zdroj:	Optics and Spectroscopy. 116:488-493
ISSN:	1562-6911 0030-400X
Popis:	Specific features of the quantitative laser microtomography of biological samples are discussed. The method exhibits the main advantages of a confocal microscope (rapid measurement of a stack of parallel optical cross sections and accurate displacement of an object along the optical axis). A relatively high contrast is reached owing to the superposition of pairwise complementary images on neighboring cross sections. A simple and convenient algorithm for image processing does not require additional software and can be computerized using a conventional graphic editor. The applicability of the method is illustrated using volume measurements of a single cell of an early mouse embryo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::51dd9b771ccfcdca7c0aeafa4d67a2ce https://doi.org/10.1134/s0030400x14030175 Zobrazit plný text záznamu Plný text ve formátu PDF