Mammalian chromosome identification in interspecific hybrid cells using 'Hoechst 33258'

Autor:	Raju Kucherlapati, Frank H. Ruddle, A. Gropp, I. Hilwig
Rok vydání:	1975
Předmět:	chemistry.chemical_compound chemistry Cell culture Chromosome Chromosomal translocation General Medicine Interspecific competition Biology Molecular biology DNA Staining
Zdroj:	Humangenetik. 27:9-14
ISSN:	0018-7348
DOI:	10.1007/bf00283498
Popis:	Treatment of fixed chromosome preparations of mouse-human hybrid cells with the benzimidazol derivative “Hoechst 33258” makes it possible to distinguish between mouse and human chromosomes. Treatment of live cells results in differential condensation of mouse and human centromeric regions. The effects of the dye on a mouse-human translocation and the species specific differences of staining and of condensation are in accordance with the assumption that the action of “H 33258” is specifically dependent on the base composition of the DNA.
Databáze:	OpenAIRE
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