Function of microtubules in intercellular transport of plant virus RNA

Autor:	Vitaly Boyko, Paul Schellenbaum, Manfred Heinlein, Jacqueline Ferralli, Jamie Ashby
Rok vydání:	2000
Předmět:	biology Microtubule viruses Intercellular transport Tobacco mosaic virus Tobamovirus Cell Biology Plasmodesma Movement protein biology.organism_classification Microtubule polymerization Conserved sequence Cell biology
Zdroj:	Nature Cell Biology. 2:826-832
ISSN:	1476-4679 1465-7392
Popis:	Cell-to-cell progression of tobacco mosaic virus (TMV) infection in plants depends on virus-encoded movement protein (MP). Here we show that a conserved sequence motif in tobamovirus MPs shares similarity with a region in tubulins that is proposed to mediate lateral contacts between microtubule protofilaments. Point mutations in this motif confer temperature sensitivity to microtubule association and viral-RNA intercellular-transport functions of the protein, indicating that MP-interacting microtubules are functionally involved in the transport of vRNA to plasmodesmata. Moreover, we show that MP interacts with microtubule-nucleation sites. Together, our results indicate that MP may mimic tubulin assembly surfaces to propel vRNA transport by a dynamic process that is driven by microtubule polymerization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::51b86f33f97ecacc806268fa6618e04b https://doi.org/10.1038/35041072 Zobrazit plný text záznamu Plný text ve formátu PDF