Enhanced Remediation of Pentachlorophenol (PCP)-Contaminated Groundwater by Bioaugmentation with Known PCP-Degrading Bacteria

Autor:	M. Lynn Prewitt, Din-Pow Ma, Hamid Borazjani, Vaibhav V. Joshi
Rok vydání:	2015
Předmět:	Chlorophenol Bioaugmentation biology Environmental remediation biology.organism_classification respiratory tract diseases Microbiology Pentachlorophenol chemistry.chemical_compound Burkholderia chemistry Contaminated groundwater Bacteria General Environmental Science Sphingobium chlorophenolicum
Zdroj:	Bioremediation Journal. 19:160-170
ISSN:	1547-6529 1088-9868
DOI:	10.1080/10889868.2014.995369
Popis:	The objective of this study was to test whether bioaugmentation with known pentachlorophenol (PCP)-degrading bacteria (Sphingobium chlorophenolicum and Burkholderia cepacia) could enhance remediation of PCP-contaminated groundwater. Groundwater PCP concentrations were determined by US Environmental Protection Agency (EPA) method 3510C and gas chromatography. Gene expression for PCP-degrading enzymes: pentachlorophenol 4-monooxygenase (pcpB; S. chlorophenolicum) and chlorophenol 4-monooxygenase (TftD; B. cepacia) was determined by real-time polymerase chain reaction (RT-PCR) using gene-specific primers. Bioaugmented treatments with S. chlorophenolicum and B. cepacia showed 32% and 49% decrease (p .05) in average PCP concentration, respectively, over 72 days. Decreased PCP levels correlated strongly (r = −.82, p < .05) with increased PCP-tolerant bacteria in bioaugmented treatments, whereas no significant co...
Databáze:	OpenAIRE
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