Using Reference Quantitative Polymerase Chain Reaction to Assess the Clinical Performance of the Paracheck-Pf® Rapid Diagnostic Test in a Field Setting in Uganda
| Autor: | Stephanie K. Yanow, Michael Hawkes, Catherine J. Mitran, Anthony K. Mbonye |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rapid diagnostic test biology business.industry 030231 tropical medicine 030106 microbiology Plasmodium falciparum Parasitemia equipment and supplies medicine.disease biology.organism_classification Virology Confidence interval 03 medical and health sciences 0302 clinical medicine Infectious Diseases Real-time polymerase chain reaction parasitic diseases Gametocyte Medicine Parasite hosting Parasitology business Malaria |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene. 99:357-359 |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Malaria rapid diagnostic tests (RDTs) are widely used in clinical and surveillance settings. However, the performance of most RDTs has not been characterized at parasite densities below detection by microscopy. We present findings from Uganda, where RDT results from 491 participants with suspected malaria were correlated with quantitative polymerase chain reaction (qPCR)-defined parasitemia. Compared with qPCR, the sensitivity and specificity of the RDT for Plasmodium falciparum mono-infections were 76% (95% confidence interval [CI]: 68-83%) and 95% (95% CI: 92-97%), respectively. The sensitivity of the RDT at parasite densities between 0.2 and 200 parasites/μL was surprisingly high (87%, 95% CI: 74-94%). The high sensitivity of the RDT is likely because of histidine-rich protein 2 from submicroscopic infections, gametocytes, or sequestered parasites. These findings underscore the importance of evaluating different RDTs in field studies against qPCR reference testing to better define the sensitivity and specificity, particularly at low parasite densities. |
| Databáze: | OpenAIRE |
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