CONCOMITANT HPLC METHOD FOR DETERMINATION OF LAMOTRIGINE, CARBAMAZEPINE, AND 10,11-CARBAMAZEPINE EPOXIDE IN PLASMA USING DUAL UV 240–220 NM WAVELENGTH DETECTION
| Autor: | A. Zerrouk, G. Dumortier, D. Januel, G. Saba, D. Pons, K. Degrassat |
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| Rok vydání: | 2001 |
| Předmět: |
Chromatography
medicine.medical_treatment Metabolite Clinical Biochemistry Pharmaceutical Science Carbamazepine Biochemistry High-performance liquid chromatography Analytical Chemistry Absorbance Drug detection chemistry.chemical_compound Anticonvulsant Blood serum chemistry medicine Quantitative analysis (chemistry) medicine.drug |
| Zdroj: | Journal of Liquid Chromatography & Related Technologies. 24:3171-3180 |
| ISSN: | 1520-572X 1082-6076 |
| DOI: | 10.1081/jlc-100107727 |
| Popis: | This paper presents a rapid, low cost, specific, high-performance liquid chromatographic (HPLC) method using an internal standard (pipamperone: PIP) for concomitant determination of lamotrigine (LMG), carbamazepine (CBZ), and its major metabolite, 10,11-carbamazepine epoxide (EPOCBZ). Drug detection is performed using a dual wavelength detection (200-240nm) to check the purity of chromatographic peaks. Mean retention times for LMG, EPOCBZ, PIP, CBZ were 3.73, 4.34, 5.41, and 7.93 minutes, respectively. Recovery ratios averaged 88.1%±1.0 for LMG, 101.1%±1.4 for CBZ, 101.0%±1.6 for EPOCBZ, and 97.2%±2.5 for PIP, respectively (n=6). In the range investigated, both the intra-day and the inter-day % relative standard deviation were less than 6.5% (n=6). Absorbance ratios of 240 versus 220 nm (AR 240/220nm) averaged 0.401±0.0037 for LMG, 0.227±0.0046 for EPOCBZ, 0.525±0.0042 for CBZ, and 3.234±0.115 for PIP, respectively (n=4). Albeit, no interference was detected with any of the drugs (or metabolites) tested, ... |
| Databáze: | OpenAIRE |
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