Influence of molar mass, dispersity, and type and location of hydrophobic side chain moieties on the critical micellar concentration and stability of amphiphilic HPMA-based polymer drug carriers
| Autor: | Sergey K. Filippov, Ekaterina A. Lomkova, Petr Chytil, Christine M. Papadakis, Eva Koziolová, Bart-Jan Niebuur, Natalya S. Vishnevetskaya, Tomáš Etrych |
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| Rok vydání: | 2017 |
| Předmět: |
Molar mass
Polymers and Plastics Dispersity 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Micelle 0104 chemical sciences chemistry.chemical_compound Colloid and Surface Chemistry chemistry Critical micelle concentration Amphiphile Polymer chemistry Materials Chemistry Side chain Methacrylamide Physical and Theoretical Chemistry 0210 nano-technology Drug carrier |
| Zdroj: | Colloid and Polymer Science. 295:1313-1325 |
| ISSN: | 1435-1536 0303-402X |
| Popis: | In the present paper, we address the question to which extent the critical micellar concentration (cmc) value can be varied for clinically related drug delivery carriers if all crucial parameters needed for successful drug delivery are already preset. For this purpose, the cmc values of an amphiphilic polymer carrier based on N-(2-hydroxypropyl) methacrylamide (HPMA) with cholesterol side groups, differing in a variety of parameters, such as molar mass, dispersity, architecture (statistical vs. diblock copolymer), and spacer structure, were studied in detail. At this, we chose physiological conditions (phosphate buffered saline at pH 7.4) and investigated the formation of micelles using fluorescence correlation spectroscopy in dependence on polymer concentration. Moreover, conjugates where the cholesterol moieties are attached to the HPMA backbone by a pH-cleavable hydrazone bond were investigated in dependence on time with respect to their long-term stability at pH 7.4, and it was found that the nanoparticles change size in the course of a few weeks. After a pH change from 7.4 to 5.0 (typical for tumor cells), the nanoparticles grow during the first few hours, then shrink. A weak correlation between the cmc value and the content of the hydrophobic group was found for HPMA-based polymers, regardless their architecture, polydispersity, and spacer structure. A much stronger correlation was observed between the cmc value and the dispersity. This finding could be explained by the higher absolute number of hydrophobic groups located on the higher molar mass fractions of the HPMA copolymers, which initiate the micelle formation. |
| Databáze: | OpenAIRE |
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