Autor:	M D Ter-Avanesyan, M O Agaphonov, So-Young Kim, Eui Sung Choi, A V Deev, Jung Hoon Sohn
Rok vydání:	2003
Předmět:	Genetics Fungal protein Saccharomyces cerevisiae Biophysics Intron Biology biology.organism_classification Yeast genomic DNA chemistry.chemical_compound chemistry Structural Biology RNA splicing Gene DNA
Zdroj:	Molecular Biology. 37:74-80
ISSN:	0026-8933
Popis:	A novel approach to isolation and functional characterization of the Hansenula polymorpha genes basing on the use of two strains of different origin is described. One of these strains is better suited for the isolation of genomic DNA fragments, while the other is preferable for their functional analysis. Thirty three genomic sequences governing expression of a reporter protein have been isolated. Analysis of the sequence encoding a homolog of the Saccharomyces cerevisiae cofilin revealed two introns. Another isolated DNA fragment encoded a homolog of the S. cerevisiae Vps10p. Disruption of the corresponding gene resulted in secretion of a vacuolar protein, carboxypeptidase Y, into the culture medium.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::51804d75cbb5d5a18dcc423e9d04b40b https://doi.org/10.1023/a:1022336914110 Zobrazit plný text záznamu Full text from SpringerLink