Goliath, a Ring-H2 Mitochondrial Protein, Regulated by Luteinizing Hormone/Human Chorionic Gonadotropin in Rat Leydig Cells1
| Autor: | Adeline Guais, Georges Guellaen, Frédérique Bulle, Brigitte Solhonne, Nathalie Melaine |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Gene isoform
endocrine system medicine.medical_specialty Leydig cell Cell Biology General Medicine Testicle Biology Sertoli cell Human chorionic gonadotropin Cell biology Immunolabeling medicine.anatomical_structure Endocrinology Reproductive Medicine Internal medicine medicine Luteinizing hormone Spermatogenesis |
| Zdroj: | Biology of Reproduction. 70:204-213 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.103.018820 |
| Popis: | We have cloned the rat homologue of the ring-H2 protein Goliath involved in Drosophila development. The rat Goliath mRNA (1.85 kb) was translated as a major ubiquitous protein species of 28-kDa and three larger isoforms (50, 46, and 36 kDa) expressed mainly in liver, lung, stomach, heart, and thymus and barely detectable in other tissues (kidney, skeletal muscle, brain, testis, intestine, and spleen). By immunohistochemistry on rat testis sections, we localized the protein in interstitial tissue and seminiferous tubules. In tubules, Goliath was expressed mainly in postmeiotic germ cells and to a much lesser extent in Sertoli cells. In the interstitium, Goliath was exclusively present in Leydig cells. Using a series of immunolabeling, cellular fractionation, and electron microscopy experiments, we established that Goliath is present in mitochondria of the R2C Leydig cell line. Using short-term hypophysectomized animals, we showed that Goliath is regulated by LH/hCG in Leydig cells but not in germ cells. This regulation in Leydig cells concerned only the 50-kDa isoform. This report is the first description of a differential regulation of the Goliath protein between germ cells and Leydig cells. |
| Databáze: | OpenAIRE |
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