PCR identification of toxic euglenid species Euglena sanguinea
| Autor: | Rafał Milanowski, Maja Łukomska-Kowalczyk, Agata Kulczycka, Bożena Zakryś |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fire ant biology Toxin 010604 marine biology & hydrobiology Fish farming Zoology Plant Science Aquatic Science medicine.disease_cause biology.organism_classification 01 natural sciences DNA barcoding Euglena sanguinea 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Euglenid Solenopsin medicine Euglenophycin |
| Zdroj: | Journal of Applied Phycology. 30:1759-1763 |
| ISSN: | 1573-5176 0921-8971 |
| DOI: | 10.1007/s10811-017-1376-z |
| Popis: | Euglena sanguinea Ehrenberg is the only known species of euglenids which forms toxic blooms causing tangible losses to fish farms. Euglena sanguinea produces euglenophycin, a toxin similar in structure to solenopsin, an alkaloid found in fire ant venom. It was proved that euglenophycin exhibits not only ichthyotoxic but also herbicidal and anticancer activity. Recently, a specific mass spectrometric method of identification and quantitation of euglenophycin was developed to facilitate monitoring of that toxin in freshwater ponds. Despite the recent taxonomic verifications, proper identification of E. sanguinea is still difficult, especially for less experienced researchers. Herein, we describe a simple method based on nested PCR amplification of the nSSU rDNA fragments to identify a single E. sanguinea cell and its detection in a sample of water. The method will further facilitate monitoring of water reservoirs, especially estimating the risk of toxic blooms. |
| Databáze: | OpenAIRE |
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