Gamma-Linolenic Acid Suppresses IgE Production in Vitro as Well as in Vivo
| Autor: | Seiji Kawamoto, Kazuhisa Ono, Seiko Shigeta, Maiko Kita, Mizuho Hamada, Osamu Suzuki, Tsunehiro Aki |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
biology
Lipopolysaccharide business.industry nutritional and metabolic diseases Stimulation Pharmacology Immunoglobulin E In vitro chemistry.chemical_compound chemistry Oral administration In vivo Splenocyte biology.protein Medicine lipids (amino acids peptides and proteins) gamma-Linolenic acid business |
| Zdroj: | Animal Cell Technology: Basic & Applied Aspects ISBN: 9789048159345 |
| DOI: | 10.1007/978-94-017-0728-2_80 |
| Popis: | Previous clinical studies have suggested that oral administration of gammalinolenic acid (GLA; C 18:3, n-6)-rich oil is effective for treatment of allergic disorders such as atopic dermatitis (AD). However, their evaluation still seems to be elusive, since contradictory clinical results have also been reported. In this study, we evaluated the effect of oral administration of purified GLA on NC/Nga mice, a recently introduced animal AD model manifesting inflammatory skin disease with hyper-IgE syndrome. We found that oral GLA administration significantly prevented hyper-IgE production. Furthermore, development of inflammatory skin lesions was significantly suppressed. These results suggest that GLA has potent preventive effect on pathogenic IgE production and the development of AD phenotype in vivo. We also found that GLA was able to suppress IgE production by NC/Nga splenocytes upon stimulation with interleukin-4 (IL-4) and lipopolysaccharide (LPS). This IgE production inhibitory effect was not observed when GLA was supplemented 72 hrs after cytokine stimulation, suggesting that GLA inhibited early stage of IgE production including class switch recombination process. |
| Databáze: | OpenAIRE |
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