| Popis: |
The porosity of agarose gels decreases as the concentration of agarose increases. However, even gels of agarose concentrations as high as 15% are permeable to macromolecules such as proteins. Spheres prerared from such high-concentration agarose gels are very rigid. Therefore, columns packed with these gel spheres permit relatively high flow-rates (particularly after cross-linking) even if the diameter of the spheres is made small in order to increase the resolution. Due to the high porosity of the gel spheres they can be used with advantage for molecular sieving of high-molecular-weight substances, for instance, biopolymers and as high-capacity matrixes for the fractionation of macromolecules by ion-exchange, affinity and hydrophobic interaction chromatography. The cost of preparing these high-concentration (cross-linked) agarose gel spheres is comparatively low, which is of particular importance when used in preparative high- or intermediate-performance liquid chromatography. The diameter of the gel spheres has been varied from 2 to 100 μm. |
