Spinach carbonic anhydrase: Identification of two immunologically related protein species
| Autor: | Pierre M. Charest, Nathalie Daigle, Peter Rogers |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Gel electrophoresis
Chromatography Chromatofocusing food and beverages Chemical modification Plant Science General Medicine Horticulture Biology biology.organism_classification Biochemistry chemistry.chemical_compound chemistry Dehydratase Carbonic anhydrase biology.protein Carbonate Spinach Chenopodiaceae Molecular Biology |
| Zdroj: | Phytochemistry. 36:1129-1132 |
| ISSN: | 0031-9422 |
| DOI: | 10.1016/s0031-9422(00)89625-9 |
| Popis: | When submitted to several chromatographic steps, carbonic anhydrase (CA: carbonate dehydratase; EC 4.2.1.1) of spinach leaves appeared as two closely spaced but distinct bands at Mr ≈ 28 × 103 as indicated by analytical SDS-polyacrylamide gel electrophoresis. Immunochemical analyses demonstrated that both peptides reacted equally with a spinach carbonic anhydrase specific antibody. Two dimensional gel immunoelectrophoretic analysis of CA indicates the presence of charge variants which may arise as a result of chemical modification. Column chromatofocusing also revealed that spinach carbonic anhydrase possesses a pI of ≈ 5.3, which is similar to animal isoforms. |
| Databáze: | OpenAIRE |
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