Antibody Guided Molecular Imaging of Infective Endocarditis
| Autor: | Barbara E. Murray, Eva M. Sevick-Muraca, Ali Azhdarinia, Kenneth L. Pinkston, Kavindra V. Singh, Barrett R. Harvey, Peng Gao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.drug_class 030106 microbiology biochemical phenomena metabolism and nutrition Biology Monoclonal antibody medicine.disease biology.organism_classification Virology Pilus Enterococcus faecalis Microbiology 03 medical and health sciences 030104 developmental biology Antigen Infective endocarditis Pilin medicine biology.protein bacteria Endocarditis Antibody |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493966714 |
| Popis: | In this protocol, we describe the application of using a high affinity monoclonal antibody generated against the major pilin protein component of the pilin structure of Enterococcus faecalis as a PET imaging agent for enterococcal endocarditis detection. The anti-pilin -mAb 64Cu conjugate was able to specifically label enterococcal endocarditis vegetation in vivo in a rodent endocarditis model. By targeting pili, a covalently linked surface antigen extending from the bacterial surface, we provided evidence that gram-positive pilin represent a logical surface antigen to define or target an infectious agent for molecularly guided imaging. Our goal in providing a detailed protocol of our efforts is to enable others to build upon this methodology to answer pertinent translational and basic research questions in the pursuit of diagnosis and treatment of infective endocarditis. |
| Databáze: | OpenAIRE |
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