Determination of the viscoelastic behavior of sodium hyaluronate in phosphate buffered saline with rheo-mechanical and rheo-optical methods
| Autor: | Werner-Michael Kulicke, D. Lohmann, F. Meyer |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Journal of Rheology. 53:799-818 |
| ISSN: | 1520-8516 0148-6055 |
| DOI: | 10.1122/1.3122985 |
| Popis: | Aqueous solutions of microbially produced sodium hyaluronate in phosphate buffered saline (pH=7.4, T=25 °C) were characterized with regard to their molecular parameters and viscoelastic behavior. The weight-average molar mass (MW), z-average radius of gyration (RG), and their distributions were determined using a hyphenated assembly of size exclusion chromatography, multi-angle light scattering, and a differential refractive index detector. Correlation of the obtained results with viscometric data enabled the establishment of [η]-M and RG-Mrelationships ([η]=0.034MW0.79; RG=0.039MW0.59). Viscoelastic behavior was investigated by steady state shear experiments. The viscosity yield was determined for varying concentrations and molar masses using shear rates from 10−3 to 103 s−1. The elastic behavior in shear flow, represented by the first normal stress difference N1, has been detected on one hand via rheo-mechanical experiments and on the other hand being correlated from the rheo-optical material functions ... |
| Databáze: | OpenAIRE |
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