Ginkgo biloba attenuated hepatotoxicity induced by combined exposure to cadmium and fluoride via modulating the redox imbalance, Bax/Bcl-2 and NF-kB signaling pathways in male rats
Autor: | Milad Arab-Nozari, Zahra Lorigooini, Fatemeh Shaki, Mohammad Shokrzadeh, Nematollah Ahangar, Fereshteh Talebpour Amiri, Ebrahim Mohammadi |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Antioxidant biology Ginkgo biloba medicine.medical_treatment Inflammation General Medicine Glutathione Pharmacology biology.organism_classification medicine.disease_cause Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry Apoptosis 030220 oncology & carcinogenesis Genetics medicine Signal transduction medicine.symptom Molecular Biology Oxidative stress |
Zdroj: | Molecular Biology Reports. 47:6961-6972 |
ISSN: | 1573-4978 0301-4851 |
Popis: | Aim of this study was to investigate the efficacy of Ginkgo Biloba (G.B) hydro-ethanolic extract against hepatotoxicity induced by combined exposure to cadmium (Cd) and fluoride (F) in Wistar rats. Animals were exposed to F (30 mg/L) + Cd (40 mg/L), F + Cd plus G.B (50,100 and 200 mg/kg), G.B (200 mg/kg), F + Cd plus Vit C(1000 mg/L) in drinking water for 42 days. Significant raise in liver enzymes and histopathological changes were observed in F + Cd treated rats. F + Cd exposure enhanced protein and glutathione oxidation, lipid peroxidation and decreased superoxide dismutase activity. F and Cd combination also caused mitochondrial dysfunction, swelling and mitochondrial membrane potential collapse in liver isolated mitochondria. Up-regulation of inflammatory genes (TNF-α, IL-1β and NF-kB) and pro-apoptotic Bax as well as down-regulation of anti-apoptotic Bcl-2 were detected after co-exposure to F and Cd. Interestingly, G.B alleviated F + Cd induced liver oxidative stress, mitochondrial damage and prevented inflammation and apoptosis. Furthermore, decrease in serum liver enzymes and improvement of histopathologic lesions were observed in G.B treated rats. This study explored the potential beneficial role of G.B on F + Cd combined hepatotoxic effects via considering its possible antioxidant, anti-inflammatory, mitochondrial protection and anti-apoptotic effects. |
Databáze: | OpenAIRE |
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